Advanced Blood Function Diagnostics

Functional Fluidics provides contract research services utilizing proprietary whole blood assays. We enable our partners to predict, select, and monitor antithrombotic therapies.

Services

We offer the following services for our pharmaceutical partners:

1. Initial Consultation

to assess possible approaches to utilizing the Functional Fluidics platform to assist in a client's drug development program.

2. Preclinical Validation

through small-scale efficacy studies, first with de-identified patient samples, and then with clinical patient samples following an existing or new IRB patient recruitment protocol.

3. Patient Selection

following preclinical validation studies, we can assist in the selection of appropriate responsive patients for a clinical study.

4. Monitoring

during a clinical study using our assays to quantify the effect effects of a drug on blood function (adhesion, thrombosis, etc) in blood samples from treated patients.

5. Companion Diagnostic Development

by partnering with our customers to develop companion diagnostics that pair the right patient, with the right treatment, at the right time.

Novel Whole Blood Diagnostic Assays

We offer the following proprietary assays:

  • Flow Adhesion

    Blood samples (whole or isolated cellular components) are pre-treated an anti-adhesive drug, then subjected to physiologic flow across an adhesive substrate of interest. Adhered cells are quantified to generate an adhesion index.

  • Flow Reverse Adhesion

    Blood cells are adhered (see Flow Adhesion) followed by introduction of anti-adhesive drug under physiologic flow. Remaining adherent cells are measured to generate a reverse adhesion index.

  • Flow Adhesion Avidity

    Blood cells are adhered (see Flow Adhesion) followed by introduction of sequentially increased shear. Remaining adherent cells are measured prior to subsequent increases in shear to generate an adhesion avidity index.

  • Flow Dynamic Adhesion

    Blood samples (whole or isolated cellular components) are pre-treated with an anti-adhesive drug, then subjected to physiologic flow across an adhesive substrate of interest. Time-lapse images are acquired to measure cell rolling / sliding along the channel surface. Mean velocity for rolling objects and cell flux is measured to generate a dynamic adhesion index.

  • Characterization of Adhesion

    Following adhesion assay (see Flow Adhesion) adhered cells are fixed with 4% formalin and stained. Fluorescence microscopy is utilized to differentiate between cell populations.
  • Flow Thrombosis

    Blood samples (whole or isolated platelets) are pre-treated with a drug of interest then subjected to physiologic arterial flow across a substrate of interest. The kinetics of thrombosis formation is measured (lag time, max rate of thrombosis, area under the curve, and maximum amplitude).

  • Flow Thrombolysis

    Blood samples (whole or isolated platelets) are pre-treated with a drug of interest then subjected to physiologic arterial flow across a substrate of interest. Thrombus is allowed to form, therapy is then added and kinetics of thrombolysis is measured to generate a thrombolysis index.

  • Membrane Fragility

    Blood samples are subjected to mechanical stress and hemolysis (cell rupture) is measured to generate a membrane fragility index.

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