Flow Adhesion
Jennell White PhD, Michael Tarasev PhD/MBA, Cidney Allen MS, Naveen Kamireddi MS, Alexander Glaros MD, Michael Callaghan MD, Ahmar Zaidi MD, Andrew D. Campbell MD, Olufunke Y. Martin MD, Asif Alavi MD, Lanetta Bronte-Hall MD, Patrick Hines MD/PhD
The objective of this study was to provide a qualitative, narrative description in deploying a specialized central lab model that focuses on RBC health biomarkers.
Michael Tarasev, Xiufeng Gao, Jennell White, Patrick Hines
Sickle cell disease (SCD) is characterized by frequent and unpredictable vaso-occlusive crises (VOCs) resulting in increased morbidity and mortality. There are no reliable biomarkers to predict the onset and progression of VOCs, complicating disease management
White J, Liu K, Gao X, Callaghan M, Hines P. A Longitudinal Study to Identify and Assess Adhesion Indices during Vaso-Occlusive Crises in Adults and Adolescents with Sickle Cell Disease. Blood 2018; 132 (Supplement 1): 1097. doi:
The decision to seek medical contact varies amongst patients. When VOCs are managed at home valuable information remains unknown. We designed a longitudinal, observational study to capture adhesion data at home and in a hospital setting. The objective of this study was to determine whether a standardized, flow-based adhesion bioassay is capable of identifying VOCs occurring in SCD patients with varying degrees of medical contact
We developed a standardized, flow-based adhesion assay to measure p-selectin mediated adhesion in SCD. Whole blood (Wb) and isolated WBC samples were perfused (1.0 dynes/cm2, 1.67Hz) through micro-fluidic channels
White J, Lancelot M, Sarnaik S, Hines P. Increased erythrocyte adhesion to VCAM-1 during pulsatile flow: Application of a microfluidic flow adhesion bioassay. Clin Hemorheol Microcirc. 2015;60(2):201-13. doi: 10.3233/CH-141847. PMID: 24898561; PMCID: PMC4923762.
Sickle cell disease (SCD) is characterized by microvascular occlusion mediated by adhesive interactions of sickle erythrocytes (SSRBCs) to the endothelium. Most in vitroflow adhesion assays measure SSRBC adhesion during continuous flow, although in vivoSSRBC adhesive interactions occur during pulsatile flow. Using a well-plate microfluidic flow adhesion system, we demonstrate that isolated SSRBCs adhere to vascular cell adhesion molecule (VCAM-1) at greater levels during pulsatile versus continuous flow.
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