--- title: Abstracts- Flow Adhesion --- [![functionalfluidics\_logo](https://www.functionalfluidics.com/hs-fs/hubfs/functionalfluidics_logo.jpg?width=132&name=functionalfluidics_logo.jpg)](https://www.functionalfluidics.com/) - [About Us](https://www.functionalfluidics.com/about-us) - [Services](https://www.functionalfluidics.com/services) - [Assays](https://www.functionalfluidics.com/assays-2) - [Flow Adhesion](https://www.functionalfluidics.com/assays/flow-adhesion) - [Mechanical Fragility](https://www.functionalfluidics.com/assays/mechanical-fragility) - [Dynamic Sickling Assay](https://www.functionalfluidics.com/assays-dynamicsicklingassay) - [Oxygenscan](https://www.functionalfluidics.com/assays/lorrca-oxygenscan) - [Osmoscan](https://www.functionalfluidics.com/assays-2/flow-adhesion-reverse-0) - [Contract Research](https://www.functionalfluidics.com/contractresearch) - [Nigeria Research Center](https://www.functionalfluidics.com/functional_fluidics_nigeria) - [RBC Test Menu](https://www.functionalfluidics.com/hubfs/Test%20Menu%20(2).pdf) - Scientific Resources - [Peer Reviewed Publications](https://www.functionalfluidics.com/scientificresources/peerreviewed) - [Abstracts](https://www.functionalfluidics.com/scientificresources/abstracts) - [ELIPSIS](https://www.functionalfluidics.com/scientificresources/elipsis) - [News](https://www.functionalfluidics.com/news-and-presentations) # Abstracts Flow Adhesion [Deployment of Red Cell Health Biomarkers at Sickle Cell Disease Pilot Sites](https://www.functionalfluidics.com/hubfs/FF%20FSCDR%20poster%202022%20updates%206-3-2022.pdf) [Read More](https://www.functionalfluidics.com/hubfs/FF%20FSCDR%20poster%202022%20updates%206-3-2022.pdf) - - - [Increased erythrocyte adhesion to VCAM-1 during pulsatile flow: Application of a microfluidic flow adhesion bioassay](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923762/pdf/ch-60-2-ch1847.pdf) [Read More](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923762/pdf/ch-60-2-ch1847.pdf) - - - FLOW ADHESION ## Deployment of Red Cell Health Biomarkers at Sickle Cell Disease Pilot Sites **Jennell White PhD, Michael Tarasev PhD/MBA, Cidney Allen MS, Naveen Kamireddi MS, Alexander Glaros MD, Michael Callaghan MD, Ahmar Zaidi MD, Andrew D. Campbell MD, Olufunke Y. Martin MD, Asif Alavi MD, Lanetta Bronte-Hall MD, Patrick Hines MD/PhD** The objective of this study was to provide a qualitative, narrative description in deploying a specialized central lab model that focuses on RBC health biomarkers. [Download PDF](https://www.functionalfluidics.com/hubfs/FF%20FSCDR%20poster%202022%20updates%206-3-2022.pdf) FLOW ADHESION ## Adhesion To VCAM-1 and P-Selectin Predict Time-To-Resolve (TTR) Of Vaso-Occlusive Crisis **Michael Tarasev, Xiufeng Gao, Jennell White, Patrick Hines** Sickle cell disease (SCD) is characterized by frequent and unpredictable vaso-occlusive crises (VOCs) resulting in increased morbidity and mortality. There are no reliable biomarkers to predict the onset and progression of VOCs, complicating disease management [Read More](https://www.functionalfluidics.com/hubfs/May-2021-Journal-Final-%2044.pdf) FLOW ADHESION ## A Longitudinal Study to Identify and Assess Adhesion Indices during Vaso-Occlusive Crises in Adults and Adolescents with Sickle Cell Disease **White J, Liu K, Gao X, Callaghan M, Hines P. A Longitudinal Study to Identify and Assess Adhesion Indices during Vaso-Occlusive Crises in Adults and Adolescents with Sickle Cell Disease. Blood 2018; 132 (Supplement 1): 1097. doi:** The decision to seek medical contact varies amongst patients. When VOCs are managed at home valuable information remains unknown. We designed a longitudinal, observational study to capture adhesion data at home and in a hospital setting. The objective of this study was to determine whether a standardized, flow-based adhesion bioassay is capable of identifying VOCs occurring in SCD patients with varying degrees of medical contact [Read More](https://ashpublications.org/blood/article/132/Supplement%201/1097/262584/A-Longitudinal-Study-to-Identify-and-Assess?searchresult=1) FLOW ADHESION ## Longitudinal Evaluation of a Standardized P-Selectin Flow Adhesion Bioassay: Potential Role for the Assessment and Prediction of Vaso-Occlusive Episodes in Sickle Cell Disease We developed a standardized, flow-based adhesion assay to measure p-selectin mediated adhesion in SCD. Whole blood (Wb) and isolated WBC samples were perfused (1.0 dynes/cm2, 1.67Hz) through micro-fluidic channels [Read More](https://ashpublications.org/blood/article/132/Supplement%201/1096/262577/Longitudinal-Evaluation-of-a-Standardized-P) FLOW ADHESION ## Increased Erythrocyte Adhesion to VCAM-1 During Pulsatile Flow: Application of a Microfluidic Flow Adhesion Bioassay **White J, Lancelot M, Sarnaik S, Hines P. Increased erythrocyte adhesion to VCAM-1 during pulsatile flow: Application of a microfluidic flow adhesion bioassay. Clin Hemorheol Microcirc. 2015;60(2):201-13. doi: 10.3233/CH-141847. PMID: 24898561; PMCID: PMC4923762.** Sickle cell disease (SCD) is characterized by microvascular occlusion mediated by adhesive interactions of sickle erythrocytes (SSRBCs) to the endothelium. Most in vitroflow adhesion assays measure SSRBC adhesion during continuous flow, although in vivoSSRBC adhesive interactions occur during pulsatile flow. Using a well-plate microfluidic flow adhesion system, we demonstrate that isolated SSRBCs adhere to vascular cell adhesion molecule (VCAM-1) at greater levels during pulsatile versus continuous flow. [Read More](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923762/pdf/ch-60-2-ch1847.pdf) ### Contact Us   # **Our Location** 440 Burroughs Street, Suite 447, Detroit, MI 48202 United States Email: [info@functionalfluidics.com](mailto:info@functionalfluidics.com) Phone:313-315-2555 ### Subscribe to Our Newsletter - [Services](https://www.functionalfluidics.com/services) - [Assays](https://www.functionalfluidics.com/assays-2) - [RBC Test Menu](https://www.functionalfluidics.com/hubfs/Test%20Menu%20(2).pdf) - [Publications](https://www.functionalfluidics.com/publications/tag/publications) © Copyright Functional Fluidics 2016.