The 7th Annual Sickle Cell Disease Therapeutics Conference

Sep 11, 2018 

| News


Functional Fluidics attended The 7th Annual Sickle Cell Disease Therapeutics Conference on September 13th in New York City.

The conference is a forum to discuss the latest advancements and future trends for treating patients with sickle cell disease. Conference attendees will hear presentations from clinical-stage companies, key opinion leaders, patients, policy makers, and healthcare analysts.

The conference was held at the Park Central Hotel in New York on September 13, 2018.

 

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No other contract research company has more experience performing blood function assays to evaluate anti-adhesive therapies in Sickle Cell Disease than Functional Fluidics.

Overview

This short video describes the origins of the company, based on research in the Hines Lab at Wayne State University. We have developed a suite of assays that address the need for better ways to assess blood function, starting with the experience of Dr. Hines as a Pediatric ICU Physician at Detroit Medical Center.

Assays At A Glance

The following is a list of standardized assays that we offer. Please click on any description for more information.

Flow Adhesion

Blood samples (whole or isolated cellular components) at baseline or following drug treatment (single dose or dose response) are subjected to physiologic flow across an adhesive substrate of interest. Adhered cells are quantified to generate an adhesion index (AI).

Flow Thrombosis

Blood samples (whole or isolated platelets) are pre-treated with a drug of interest, and then subjected to physiologic arterial flow across a substrate of interest. A series of photomicrographs of fluorescently labeled blood (see Figure 3) is analyzed to determine the kinetics of thrombosis formation (lag time, max rate of thrombosis, area under the curve, and maximum amplitude).

Flow Adhesion Dynamic

Blood samples (whole or isolated cellular components) at baseline or following drug treatment (single dose or dose response) are subjected to physiologic flow across an adhesive substrate of interest (usually selectins). Time-lapse images are acquired to measure cell rolling / sliding along the channel surface. Mean velocity for rolling objects and cell flux is measured to generate a dynamic adhesion index (dAI).

Flow Adhesion Dynamic

Blood cells are adhered to a substrate of interest (see Flow Adhesion), followed by introduction of anti-adhesive drug under flow. Remaining adherent cells are measured to generate a reverse adhesion index (rAI).

Flow Adhesion Characterization

Adhered cells are fixed with 4% formalin and stained following an adhesion assay (FF-FA, FF-FAR, FF-FAA). Fluorescence microscopy is utilized to differentiate between and quantify specific cell populations.

Flow Adhesion Avidity

Blood cells are adhered to a substrate of interest (see Flow Adhesion), followed by introduction of sequentially increased shear. Remaining adherent cells are quantified followed by a sequential increase in shear (5, 10, and 20 dyne/cm^2), to generate an avidity adhesion index (aAI).

Gallery

Contact Us

Our Location

440 Burroughs Street,
Suite 641
Detroit, MI
48202
United States

Email: john@functionalfluidics.com
Phone: 734-418-8139

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