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Functional Fluidics will be attending the American Society of Hematology December 1-4 in San Diego

 

Please visit us in the Exhibit Hall: Booth 1257 

 

And Oral and Poster Abstracts:

Session: 114. Hemoglobinopathies, Excluding Thalassemia—Clinical: Poster I
Hematology Disease Topics & Pathways:
Saturday, December 1, 2018, 6:15 PM-8:15 PM
Hall GH (San Diego Convention Center)

 

Poster 1096: Longitudinal Evaluation of a Standardized P-Selectin Flow Adhesion Bioassay: Potential Role for the Assessment and Prediction of Vaso-Occlusive Episodes in Sickle Cell Disease

 

Poster 1097: A Longitudinal Study to Identify and Assess Adhesion Indices during Vaso-Occlusive Crises in Adults and Adolescents with Sickle Cell Disease

What if you  monitor the red cell health of your patients? Functional Fluidics is developing biomarkers and surrogate endpoints for sickle cell disease.

 

Overview

This short video describes the origins of the company, based on research in the Hines Lab at Wayne State University. We have developed a suite of assays that address the need for better ways to assess blood function, starting with the experience of Dr. Hines as a Pediatric ICU Physician at Detroit Medical Center.

Assays At A Glance

The following is a list of standardized assays that we offer. Please click on any description for more information.

Flow Adhesion

Blood samples (whole or isolated cellular components) at baseline or following drug treatment (single dose or dose response) are subjected to physiologic flow across an adhesive substrate of interest. Adhered cells are quantified to generate an adhesion index (AI).

Flow Thrombosis

Blood samples (whole or isolated platelets) are pre-treated with a drug of interest, and then subjected to physiologic arterial flow across a substrate of interest. A series of photomicrographs of fluorescently labeled blood (see Figure 3) is analyzed to determine the kinetics of thrombosis formation (lag time, max rate of thrombosis, area under the curve, and maximum amplitude).

Flow Adhesion Dynamic

Blood samples (whole or isolated cellular components) at baseline or following drug treatment (single dose or dose response) are subjected to physiologic flow across an adhesive substrate of interest (usually selectins). Time-lapse images are acquired to measure cell rolling / sliding along the channel surface. Mean velocity for rolling objects and cell flux is measured to generate a dynamic adhesion index (dAI).

Flow Adhesion Reverse 

Blood cells are adhered to a substrate of interest (see Flow Adhesion), followed by introduction of anti-adhesive drug under flow. Remaining adherent cells are measured to generate a reverse adhesion index (rAI).

Flow Adhesion Characterization 

Adhered cells are fixed with 4% formalin and stained following an adhesion assay. Fluorescence microscopy is utilized to differentiate between and quantify specific cell populations.

Flow Adhesion Avidity

Blood samples (whole or isolated cellular components) at baseline or following drug treatment (single dose or dose response) are subjected to physiologic flow across an adhesive substrate of interest (usually selectins). Time-lapse images are acquired to measure cell rolling / sliding along the channel surface. Mean velocity for rolling objects and cell flux is measured to generate a dynamic adhesion index (dAI).