--- title: Novel Epinephrine and Cyclic AMP-Mediated Activation of B-CAM/Lu-Dependent Sickle (SS) RBC Adhesion. description: Novel Epinephrine and Cyclic AMP-Mediated Activation of B-CAM/Lu-Dependent Sickle (SS) RBC Adhesion. image: https://www.functionalfluidics.com/hubfs/Screenshot%202018-09-27%2016.45.39.png --- [![functionalfluidics_logo](https://www.functionalfluidics.com/hs-fs/hubfs/functionalfluidics_logo.jpg?width=132&name=functionalfluidics_logo.jpg)](https://www.functionalfluidics.com/) - [About Us](https://www.functionalfluidics.com/about-us) - [Services](https://www.functionalfluidics.com/services) - [Assays](https://www.functionalfluidics.com/assays-2) - [Flow Adhesion](https://www.functionalfluidics.com/assays/flow-adhesion) - [Mechanical Fragility](https://www.functionalfluidics.com/assays/mechanical-fragility) - [Dynamic Sickling Assay](https://www.functionalfluidics.com/assays-dynamicsicklingassay) - [Oxygenscan](https://www.functionalfluidics.com/assays/lorrca-oxygenscan) - [Osmoscan](https://www.functionalfluidics.com/assays-2/flow-adhesion-reverse-0) - [Contract Research](https://www.functionalfluidics.com/contractresearch) - [Nigeria Research Center](https://www.functionalfluidics.com/functional_fluidics_nigeria) - [RBC Test Menu](https://www.functionalfluidics.com/hubfs/Test%20Menu%20(2).pdf) - Scientific Resources - [Peer Reviewed Publications](https://www.functionalfluidics.com/scientificresources/peerreviewed) - [Abstracts](https://www.functionalfluidics.com/scientificresources/abstracts) - [ELIPSIS](https://www.functionalfluidics.com/scientificresources/elipsis) - [News](https://www.functionalfluidics.com/news-and-presentations) [< Go Back To Publications](https://www.functionalfluidics.com/publications/) ## Novel Epinephrine and Cyclic AMP-Mediated Activation of B-CAM/Lu-Dependent Sickle (SS) RBC Adhesion. Novel Epinephrine and Cyclic AMP-Mediated Activation of B-CAM/Lu-Dependent Sickle (SS) RBC Adhesion. - - - [Publications](https://www.functionalfluidics.com/publications/topic/publications), [Adhesion](https://www.functionalfluidics.com/publications/topic/adhesion), [Sickle Cell Disease](https://www.functionalfluidics.com/publications/topic/sickle-cell-disease) April 15, 2003 0% read Subscribe to our Newsletter ###### **Journal: **[Blood](http://www.ncbi.nlm.nih.gov/pubmed/12506027) 15 APRIL 2003  VOLUME 101, NUMBER 8  [Dowload full article with this link](https://www.functionalfluidics.com/hubfs/Novel%20epinephrine%20and%20cyclic%20AMP-mediated%20activation%20of%20BCAMLu-dependent%20sickle%20(SS)%20RBC%20adhesion.pdf) **Hines PC**, Zen Q, Burney SN, Shea DI, Ataga KA, Orringer EP, Telen MJ, Parise LV. ### Abstract The vasoocclusive crisis is the major clinical feature of sickle cell anemia, which is believed to be initiated or sustained by sickle (SS) red blood cell (RBC) adhesion to the vascular wall. SS RBCs, but not unaffected (AA) RBCs, adhere avidly to multiple components of the vascular wall, including laminin. Here we report a novel role for epinephrine and cyclic adenosine monophosphate (cAMP) in the regulation of human SS RBC adhesiveness via the laminin receptor, basal cell adhesion molecule/Lutheran (BCAM/Lu). Our data demonstrate that peripheral SS RBCs contain greater than 4-fold more cAMP than AA RBCs under basal conditions. Forskolin or the stress mediator epinephrine further elevates cAMP in SS RBCs and increases adhesion of SS RBCs to laminin in a protein kinase A (PKA)-dependent manner, with the low-density population being the most responsive. Epinephrine-stimulated adhesion to laminin, mediated primarily via the beta 2-adrenergic receptor, occurred in SS RBC samples from 46% of patients and was blocked by recombinant, soluble BCAM/Lu, implicating this receptor as a target of cAMP signaling. Thus, these studies demonstrate a novel, rapid regulation of SS RBC adhesion by a cAMP-dependent pathway and suggest that components of this pathway, particularly PKA, the beta 2-adrenergic receptor, and BCAM/Lu, should be further explored as potential therapeutic targets to inhibit SS RBC adhesion. PMID: [12506027](http://www.ncbi.nlm.nih.gov/pubmed/12506027) DOI: [10.1182/blood-2001-12-0289](http://dx.doi.org/10.1182/blood-2001-12-0289) Subscribe to our Newsletter Subscribe to our Newsletter ### ### Recommended Reads ![](https://www.functionalfluidics.com/hubfs/Screenshot%202025-12-01%20105529.png) Dec 1, 2025 #### [Functional Fluidics Welcomes Biopharma Leader Bert Bruce as Senior Advisor](https://www.functionalfluidics.com/publications/functional-fluidics-welcomes-biopharma-leader-bert-bruce-as-senior-advisor) ![](https://www.functionalfluidics.com/hubfs/Screenshot%202025-11-30%20195918.png) Dec 1, 2025 #### [Functional Fluidics to Present New Sickle Cell Disease Data at the 2025 ASH Annual Meeting in Orlando, Florida](https://www.functionalfluidics.com/publications/functional-fluidics-to-present-new-sickle-cell-disease-data-at-the-2025-ash-annual-meeting-in-orlando-florida) ### Contact Us   # **Our Location** 440 Burroughs Street, Suite 447, Detroit, MI 48202 United States Email: [info@functionalfluidics.com](mailto:info@functionalfluidics.com) Phone:313-315-2555 ### Subscribe to Our Newsletter - [Services](https://www.functionalfluidics.com/services) - [Assays](https://www.functionalfluidics.com/assays-2) - [RBC Test Menu](https://www.functionalfluidics.com/hubfs/Test%20Menu%20(2).pdf) - [Publications](https://www.functionalfluidics.com/publications/tag/publications) © Copyright Functional Fluidics 2016.