--- title: "Sickle Cell Adhesion to Laminin: Potential Role for the alpha5 Chain." description: "Sickle Cell Adhesion to Laminin: Potential Role for the alpha5 Chain." --- [![functionalfluidics\_logo](https://www.functionalfluidics.com/hs-fs/hubfs/functionalfluidics_logo.jpg?width=132&name=functionalfluidics_logo.jpg)](https://www.functionalfluidics.com/) - [About Us](https://www.functionalfluidics.com/about-us) - [Services](https://www.functionalfluidics.com/services) - [Assays](https://www.functionalfluidics.com/assays-2) - [Flow Adhesion](https://www.functionalfluidics.com/assays/flow-adhesion) - [Mechanical Fragility](https://www.functionalfluidics.com/assays/mechanical-fragility) - [Dynamic Sickling Assay](https://www.functionalfluidics.com/assays-dynamicsicklingassay) - [Oxygenscan](https://www.functionalfluidics.com/assays/lorrca-oxygenscan) - [Osmoscan](https://www.functionalfluidics.com/assays-2/flow-adhesion-reverse-0) - [Contract Research](https://www.functionalfluidics.com/contractresearch) - [Nigeria Research Center](https://www.functionalfluidics.com/functional_fluidics_nigeria) - [RBC Test Menu](https://www.functionalfluidics.com/hubfs/Test%20Menu%20(2).pdf) - Scientific Resources - [Peer Reviewed Publications](https://www.functionalfluidics.com/scientificresources/peerreviewed) - [Abstracts](https://www.functionalfluidics.com/scientificresources/abstracts) - [ELIPSIS](https://www.functionalfluidics.com/scientificresources/elipsis) - [News](https://www.functionalfluidics.com/news-and-presentations) [\< Go Back To Publications](https://www.functionalfluidics.com/publications/) ## Sickle Cell Adhesion to Laminin: Potential Role for the alpha5 Chain. Sickle Cell Adhesion to Laminin: Potential Role for the alpha5 Chain. - - - [Publications](https://www.functionalfluidics.com/publications/topic/publications) October 15, 1998 0% read Subscribe to our Newsletter [Blood 92](http://www.ncbi.nlm.nih.gov/pubmed/9763582) Lee SP, Cunningham ML, **Hines PC**, Joneckis CC, Orringer EP, Parise LV. ### Abstract Sickle red blood cell (RBC) adhesion to the endothelium and to exposed, underlying subendothelial proteins is believed to contribute to vascular occlusion in sickle cell disease. Laminin, a major component of the subendothelium, supports significant adhesion of sickle, but not normal RBCs. The purpose of this study was to define the adhesive region for sickle RBCs within a human laminin preparation using a flow adhesion assay designed to mimic physiologic flow through postcapillary venules. Because sickle RBCs did not adhere to the common laminin contaminants entactin or collagen type IV, neither of these proteins are likely to contribute to the observed adhesion to laminin. Known adhesive regions of laminin neither supported nor inhibited sickle RBC adhesion to laminin, suggesting a mechanism of adhesion previously uncharacterized in other laminin adhesion studies. Moreover, sickle RBCs did not adhere to mouse EHS laminin or to human laminin-2 (merosin), eliminating the alpha1, alpha2, beta1, and gamma1 chains as mediators of sickle cell adhesion. The monoclonal antibody 4C7, which binds at or near the G-domain of the laminin alpha5 chain, significantly inhibited sickle RBC adhesion. These results suggest that an adhesive region for sickle RBCs is contained within the laminin alpha5 chain. Copyright 1998 by The American Society of Hematology. PMID: [9763582](http://www.ncbi.nlm.nih.gov/pubmed/9763582) Subscribe to our Newsletter Subscribe to our Newsletter ### ### Recommended Reads ![](https://www.functionalfluidics.com/hubfs/Screenshot%202025-12-01%20105529.png) Dec 1, 2025 #### [Functional Fluidics Welcomes Biopharma Leader Bert Bruce as Senior Advisor](https://www.functionalfluidics.com/publications/functional-fluidics-welcomes-biopharma-leader-bert-bruce-as-senior-advisor) ![](https://www.functionalfluidics.com/hubfs/Screenshot%202025-11-30%20195918.png) Dec 1, 2025 #### [Functional Fluidics to Present New Sickle Cell Disease Data at the 2025 ASH Annual Meeting in Orlando, Florida](https://www.functionalfluidics.com/publications/functional-fluidics-to-present-new-sickle-cell-disease-data-at-the-2025-ash-annual-meeting-in-orlando-florida) ### Contact Us   # **Our Location** 440 Burroughs Street, Suite 447, Detroit, MI 48202 United States Email: [info@functionalfluidics.com](mailto:info@functionalfluidics.com) Phone:313-315-2555 ### Subscribe to Our Newsletter - [Services](https://www.functionalfluidics.com/services) - [Assays](https://www.functionalfluidics.com/assays-2) - [RBC Test Menu](https://www.functionalfluidics.com/hubfs/Test%20Menu%20(2).pdf) - [Publications](https://www.functionalfluidics.com/publications/tag/publications) © Copyright Functional Fluidics 2016.